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SAB4200804

Sigma-Aldrich

Anti-Goat/sheep IgG antibody, Mouse monoclonal

clone GT-34, purified from hybridoma cell culture

同義詞:

Goat/Sheep immunoglobulin

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

GT-34, monoclonal

形狀

buffered aqueous solution

物種活性

goat, sheep

包裝

antibody small pack of 25 μL

濃度

~1 mg/mL

技術

indirect ELISA: 0.25-0.5 μg/mL using 5μg/ml Goat IgG for coating.

同型

IgG1

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

Goat and sheep antibodies are widely used as primary antibodies against many antigens in various assay techniques in research and clinical practices. Polyclonal second antibodies to goat and sheep may suffer from a lack of unique specificity to goat or sheep IgGs, and may cross react with other IgGs from human, mouse, rat or rabbit origin. As a result, an extensive adsorbing stage has to be integrated in the manufacturing process of most polyclonal antibodies. The use of a monoclonal antibody specific to goat or sheep IgG which is devoid of any binding capacity to human and many other species, can therefore serve as an essential tool and a useful control in many tests and applications.
Monoclonal Anti-Goat/sheep IgG (mouse IgG1 isotype) is derived from the GT-34 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified goat immunoglobulins (IgGs). Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.

特異性

Monoclonal Anti-Goat/sheep recognizes an epitope located on the heavy chain of goat IgG1 and IgG2. The antibody shows strong cross-reactivity with sheep IgG and weak cross-reactivity with bovine IgG. No cross-reactivity is observed with IgG derived from human, monkey, guinea pig, rat, horse, fish, donkey, dog, chicken, pig, mouse, rabbit or cat origin. The antibody is recommended to use in various immunological techniques, including ELISA, Immunoblotting, immunofluorescence and Immunohistochemistry.

免疫原

purified goat immunoglobulins (IgGs).

生化/生理作用

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

外觀

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

其他說明

This product is for R&D use only, not for drug, household, or other uses.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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