SAB4200750

Anti-Gelsolin antibody, Mouse Monoclonal

clone GS-2C4, purified from hybridoma cell culture

• 同義詞: Anit-AGEL, Anit-Actin-depolymerizing factor (ADF), Anit-GSN
• 分類程式碼代碼: 12352203
• NACRES: NA.41
• 無性繁殖: GS-2C4, monoclonal
• application: IF; IHC; IP
• 物種活性: rabbit, canine, human, mouse, monkey
• 技術: immunoblotting: 1-2.5 μg/mL using MDCK cell line extract; immunofluorescence: suitable; immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections; immunoprecipitation (IP): suitable
• citations: 6

屬性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: GS-2C4, monoclonal
• 形狀: buffered aqueous solution
• 分子量: ~85 kDa
• 物種活性: rabbit, canine, human, mouse, monkey
• 濃度: ~1.0 mg/mL
• 技術: immunoblotting: 1-2.5 μg/mL using MDCK cell line extract; immunofluorescence: suitable; immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections; immunoprecipitation (IP): suitable
• 同型: IgG1
• UniProt登錄號: P06396
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GSN(2934)

描述

一般說明

Anti-Gelsolin Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GS-2C4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Gelsolin operates in at least two variant forms encoded by a single gene and is located both intracellular (in cytosol and mitochondria) and extracellular (in blood plasma).

免疫原

Human plasma gelsolin

應用

Anti-Gelsolin antibody, Mouse Monoclonal has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry
• immunoprecipitation

生化／生理作用

Gelsolin, also known as Actin-depolymerizing factor (ADF), GSN, AGEL or Brevin, is a calcium-dependent actin-binding protein. Gelsolin acts as a main modulator of actin filament formation and disassembly and is involved in a variety of cellular mechanisms including apoptosis, signal transduction, transcriptional regulation and epigenetic processes. Plasma gelsolin differs in its sequence from the cytoplasmic variant in possessing a 25-amino acid N-terminal extension. The main function of the cytoplasmic gelsolin is to remodel the actin cytoskeleton. Plasma gelsolin′s main role is to scan for actin fibrils that are released by injured tissue into the bloodstream and to bind them in a scavenger mode preventing actin from increasing the blood viscosity. Gelsolin levels correlate with inflammation and several diseases and pathologies, including cancer, infection, cardiac injury, pulmonary diseases, Alzheimer′s disease and aging thus making it a potential biomarker of diagnostic and prognostic values.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable