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Merck
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重要文件

SAB4200743

Sigma-Aldrich

Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal

clone HP-6017, purified from hybridoma cell culture

同義詞:

Anti-Immunoglubulin G (Fc specific)

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

品質等級

共軛

peroxidase conjugate

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

HP-6017, monoclonal

形狀

lyophilized powder

物種活性

human

濃度

~2 mg/mL

技術

ELISA: 1:80,000-1:160,000 using 5 μg/mL Human IgG myeloma for coating

同型

IgG2a

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the HP-6017 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG myeloma proteins covalently coupled to poly aminostyrene (PAS) microbeads. IgGs (Immunoglobulin G) are the most common Immunoglobulin′s isotype in blood, lymph, cerebrospinal and peritoneal fluids and serve as key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), consist of a variable Fab fragment (which includes the antibody recognition site) and a conserved Fc fragment.

免疫原

Human IgG myeloma proteins

應用

Anti-Human IgG (Fc specific)-Peroxidase antibody has been used in enzyme linked immunosorbent assay (ELISA).

生化/生理作用

IgG (Immunoglobulin G) deficiencies are often associated with various diseases. The Fc fragment has various important functions (e.g complement fixation, site for rheumatoid factor attachment and protein A binding) which indicate on the importance of immunoreagents specific for the Fc fragment of Human IgG.

外觀

Supplied as a lyophilized powder.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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N Ronda et al.
Clinical and experimental immunology, 109(1), 211-216 (1997-07-01)
Interactions between circulating IgG and endothelial cells (EC) in humans have been described only in conditions associated with pathologic immunoglobulins and/or activated or damaged EC. In this study we provide evidence that normal human IgG includes one/some antibody species that
R Jefferis et al.
Immunology letters, 10(3-4), 223-252 (1985-01-01)
Seventy-four monoclonal antibodies (McAb) of putative specificity for human IgG (11), the IgG sub-classes (59) or Gm allotypes (4) have been evaluated for reactivity and specificity in eight laboratories employing different assay techniques or protocols. For the IgG, IgG3, IgG4
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Vaccine, 33(49), 6988-6996 (2015-09-22)
Vaccination is at present the most efficient way of preventing influenza infections. Currently used inactivated influenza vaccines can induce virus-neutralizing antibodies that are protective against a particular influenza strain, but hamper the induction of cross-protective T-cell responses to later infections.
R Jefferis et al.
Annales de biologie clinique, 52(1), 57-65 (1994-01-01)
Secondary systemic immune responses are predominantly of the IgG class and passive administration of intravenous IgG, from pooled normal serum, is an effective prophylactic and/or therapeutic treatment for patients with defined immunodeficiencies. However, the proportions of each IgG subclass present

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