推薦產品
生物源
mouse
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
AGO2-10, monoclonal
形狀
buffered aqueous solution
分子量
~95 kDa
物種活性
human
濃度
~1.0 mg/mL
技術
immunoblotting: 2-4 μg/mL using extract of HEK-293T cells transfected with human AGO2
immunoprecipitation (IP): 10-20 μg/test using HeLa cells extract
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... AGO2(27161)
一般說明
抗-AGO2小鼠单克隆抗体（小鼠IgG1同工型）来源于用人AGO2蛋白C-末端区域并与匙孔血蓝蛋白（KLH）偶联的合成肽免疫的BALB/c小鼠骨髓瘤细胞，和脾细胞相互融合而产生的AGO2-10杂交瘤细胞。AGO2也称为蛋白argonaute 2、argonaute RNA诱导的沉默复合物（RISC）催化成分2、真核翻译起始因子2C 2（eIF2C2）和PAZ Piwi域蛋白（PPD）。Argonaute 2（AGO2）属于Argonaute家族。它定位于人类染色体8q24。Ago蛋白定位于体细胞的细胞质，并在细胞质加工体中浓缩。
免疫原
对应于人AGO2蛋白C末端区域的合成肽，其与KLH偶联。
應用
抗-AGO2小鼠单克隆抗体已被用于免疫印迹和免疫沉淀。
生化／生理作用
Argonaute 2（AGO2）具有内在的核酸内切酶活性。它能以小RNA向导的方式直接切割mRNA。AGO2可作为RNA诱导的沉默复合物（RISC）的必要催化成分在RNA沉默过程中起核心作用。它对于稳定细胞外体液中外泌体中的循环microRNA（miRNA）中具有重要作用。
外觀
溶于含有 15 mM 叠氮化钠的 0.01M 磷酸盐缓冲盐水（pH 7.4）溶液中。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Lasse Peters et al.
Molecular cell, 26(5), 611-623 (2007-06-15)
Small regulatory RNAs such as short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi interacting RNAs (piRNAs) have been discovered in the past, and it is becoming more and more apparent that these small molecules have key regulatory functions. Small RNAs
Andrey Turchinovich et al.
Methods in molecular biology (Clifton, N.J.), 1024, 97-107 (2013-05-31)
Circulating microRNAs (miRNAs) have been recently detected in extracellular body fluids and proved themselves as promising biomarkers for a broad spectrum of diseases. The techniques to isolate, detect, and characterize extracellular miRNAs vary significantly from report to report. In this
Jidong Liu et al.
Science (New York, N.Y.), 305(5689), 1437-1441 (2004-07-31)
Gene silencing through RNA interference (RNAi) is carried out by RISC, the RNA-induced silencing complex. RISC contains two signature components, small interfering RNAs (siRNAs) and Argonaute family proteins. Here, we show that the multiple Argonaute proteins present in mammals are
Argonaute2 promotes tumor metastasis by way of up-regulating focal adhesion kinase expression in hepatocellular carcinoma
Cheng N, et al.
Hepatology, 57(5), 1906-1918 (2013)
Shao-Chun Wu et al.
PloS one, 8(10), e77936-e77936 (2013-11-10)
The levels of circulating microRNAs (miRNAs) in mice with experimental sepsis induced by cecal ligation and puncture (CLP) were determined using whole blood samples obtained from C57BL/6 mice at 4, 8, and 24 h after CLP; miRNA expression analysis was
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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