生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
P5D4, monoclonal
形狀
buffered aqueous solution
物種活性
virus (Vesicular stomatitis virus (VSV))
包裝
antibody small pack of 25 μL
濃度
~1 mg/mL
技術
immunoblotting: 0.5-1 μg/mL using whole extract of human HEK-293T cells over-expressing Vinculin with VSV-G tagged fusion protein.
immunocytochemistry: suitable
immunofluorescence: 5-10 μg/mL using COS7 cells over-expressing Vinculin with VSV-G tagged fusion protein.
immunoprecipitation (IP): suitable
同型
IgG1
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
一般說明
小鼠单克隆抗VSV糖蛋白抗体（小鼠IgG1同种型）衍生自杂交瘤P5D4，该杂交瘤是通过小鼠骨髓瘤细胞与经水泡性口腔炎病毒糖蛋白（VSV-G）的合成肽（与KLH缀合）免疫的BALB/c小鼠脾细胞融合而产生的。
應用
小鼠抗VSV糖蛋白单克隆抗体可用于免疫印迹、免疫荧光、免疫沉淀、免疫细胞化学、透射电镜（TEM）和应用显微注射抗体的研究中。
生化／生理作用
水泡性口腔炎病毒糖蛋白（VSV-G）可构建一个具有吸引力的模型，从而用于研究膜蛋白的成熟和细胞内运输。它可介导VSV附着在细胞表面，并诱导病毒和靶膜之间的pH依赖性融合。此外，它还具有在其胞质域内进行细胞内分选的信息，包括从内质网（ER）高效输出、基底外侧传递和胞吞作用。高尔基体池之间的单向运输已使用含有VSV-G的高尔基体膜进行了证明。VSV-G的温度敏感突变体可用于研究内质网折叠中间体的存在。
外觀
溶于含有 15 mM 叠氮化钠的 0.01M 磷酸盐缓冲盐水（pH 7.4）溶液中。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
C Hammond et al.
The Journal of cell biology, 126(1), 41-52 (1994-07-01)
Proteins synthesized in the ER are generally transported to the Golgi complex and beyond only when they have reached a fully folded and assembled conformation. To analyze how the selective retention of misfolded proteins works, we monitored the long-term fate
T E Kreis et al.
Cell, 46(6), 929-937 (1986-09-12)
Using ts045, a temperature sensitive strain of Vesicular stomatitis virus, we show that oligomerization of G protein is a prerequisite for its transport from RER to the Golgi apparatus and for its subsequent maturation. While wild-type G forms an oligomer
T Soldati et al.
Cell, 66(2), 277-289 (1991-07-26)
The isoprotein-specific intracompartmental sorting of the three essential myosin light chains (LCs), the skeletal muscle LC-1f and LC-3f and the nonmuscle LC-3nm, was investigated. Epitope tagging was used to monitor the intracellular localization to different cytoskeletal structures of the exogenously
p38: A novel protein that associates with the vesicular stomatitis virus glycoprotein
Sevier CS and Machamer CE
Biochemical and Biophysical Research Communications, 287(2), 574-582 (2001)
C S Sevier et al.
Molecular biology of the cell, 11(1), 13-22 (2000-01-19)
The vesicular stomatitis virus (VSV) G protein is a model transmembrane glycoprotein that has been extensively used to study the exocytotic pathway. A signal in the cytoplasmic tail of VSV G (DxE or Asp-x-Glu, where x is any amino acid)
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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