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SAB4200673

Sigma-Aldrich

Monoclonal Anti-α-Amylase 1A antibody produced in mouse

clone AMY-7, purified from hybridoma cell culture

同義詞:

AMY1, AMY1A, alpha 1A (salivary), amylase

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

AMY-7, monoclonal

形狀

buffered aqueous solution

分子量

~58 kDa

物種活性

human, rat, mouse

濃度

~1 mg/mL

技術

immunoblotting: 0.25-0.5 μg/mL using rat pancreas tissue extracts.
immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections.

同型

IgG1

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... AMY1A(276)
mouse ... Amy1(11722)
rat ... Amy1a(24203)

一般說明

Monoclonal Anti- α-Amylase 1A (mouse IgG1 isotype) is derived from the hybridoma AMY-7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an N-terminal His-tagged recombinant human α. α-Amylase 1A, also known as AMY1A belongs to the α-Amylase family. AMY1A is a secreted digestive enzyme produced by the salivary glands and pancreas.

免疫原

N-terminal His-tagged recombinant human α-Amylase 1A.

應用

Monoclonal Anti-α-Amylase 1A antibody has been used in immunoblotting, immunohistochemistry.

生化/生理作用

α-Amylase 1A cleaves the glycosidic linkages in starch molecules to produce smaller saccharides such as maltotriose, maltose, and small amounts of glucose. AMY1A has been identified as an indicator in pancreas and salivary gland diseases, as well as lung cancer, ovarian cancer, plasmacytoma and thyroid adenomas.

外觀

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Expression of alpha-amylase isozymes in human thyroid tissues
Doi S, et al.
Cancer research, 51(13), 3544-3549 (1991)
Doi, S., et al.,
Cancer Research, 51, 3544-3549 (1991)
Paola Serrano Martinez et al.
Oral diseases, 27(1), 52-63 (2020-06-13)
Hyposalivation-related xerostomia is an irreversible, untreatable, and frequent condition after radiotherapy for head and neck cancer. Stem cell therapy is an attractive option of treatment, but demands knowledge of stem cell functioning. Therefore, we aimed to develop a murine parotid
Ramasubbu, N., et al.,
Acta Crystallographica, 52, 435-446 (1996)
Subsite mapping of human salivary alpha-amylase and the mutant Y151M
Kandra L, et al.
Febs Letters, 544(1-3), 194-198 (2003)

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