SAB4200606

Anti-EB3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 同義詞: Anti-EBF3, Anti-EBF3-S, Anti-MAPRE3, Anti-RP/EB family, Anti-RP3, Anti-member 3, Anti-microtubule-associated protein
• 分類程式碼代碼: 12352203
• NACRES: NA.41
• 共軛: unconjugated
• application: IP
• 無性繁殖: polyclonal
• 物種活性: mouse, human, rat
• citations: 7
• 技術: immunoblotting: 1-2 μg/mL using lysates of rat brain (S1 fraction); immunoprecipitation (IP): 5-10 μg using lysates of mouse brain (S1 fraction).

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~32 kDa
• 物種活性: mouse, human, rat
• 濃度: ~1.0 mg/mL
• 技術: immunoblotting: 1-2 μg/mL using lysates of rat brain (S1 fraction); immunoprecipitation (IP): 5-10 μg using lysates of mouse brain (S1 fraction).
• UniProt登錄號: Q9UPY8
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MAPRE3(22924)

描述

一般說明

End-binding protein-3 (EB3), also known as microtubule-associated protein RP/EB 3 (MAPRE3), belongs to a distinct family of “plus-end tracking proteins” (+TIPs). EB proteins are among the most conserved components of the microtubules cytoskeleton. EB proteins contain an N-terminal calponin homology (CH) domain and a unique EB-like C-terminal motif. EB3 is exclusively expressed in the central nervous system and it interacts with a CNS-specific APC homolog APCL.

免疫原

Synthetic peptide corresponding to an internal sequence of human EB3, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse EB3.

應用

Anti-EB3 antibody produced in rabbit has been used in:

• immunoblotting
• co-immunoprecipitation
• immunocytochemistry
• glutathione S-transferase (GST) pull-down assay

生化／生理作用

In neurons, End-binding protein-3 (EB3) has been shown to interact with scaffold protein ankyrin-G and PSD95 and is required for stabilizing neuronal polarity in mature neurons and in determining neuronal dendritic morphology. EB3 is also involved in MT polymerization and cell migration. EB3 may be involved in stabilizing MTs and anchoring them to centrosomes during spindle formation. Knockout of EB3 inhibits myoblasts elongation and fusion into myotubes.

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable