SAB4200461

Anti-Arc (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 同義詞: Anti-Activity-regulated cytoskeleton-associated protein, Anti-Arc3.1, Anti-Arg3.1
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~55 kDa
• 物種活性: rat, human
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.0 mg/mL
• 技術: immunohistochemistry: 20-30 μg/mL using formalin-fixed, paraffin-embedded rat brain.; immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells over-expressing human Arc.; western blot: 1.5-3.0 μg/mL using extracts of HEK-293T cells over-expressing human Arc.
• UniProt登錄號: Q7LC44
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ARC(23237) ; rat ... Arc(54323)

描述

一般說明

Activity-regulated cytoskeleton-associated protein (Arc) is a growth factor and immediate-early gene (IEG) that is enriched in the brain. Arc is located in the distal dendrites and spine. The Arc gene is located on the human chromosome at 8q24.3.

特異性

Anti-Arc(N-terminal) specifically recognizes humanand rat Arc.

免疫原

synthetic peptide corresponding to a sequence at the N-terminal region of human Arc, conjugated to KLH. The corresponding sequence is highly conserved (90% sequence identity) in rat and mouse Arc.

應用

Anti-Arc (N-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry

生化／生理作用

Activity-regulated cytoskeleton-associated protein (Arc) induction is required for late long-term potentiation (LTP) and memory consolidation. It also plays a role in LTP-induced cofilin phosphorylation and F-actin stabilization. This protein affects synaptic plasticity. Arc regulates the endocytosis of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors through its interaction with endocytic proteins endophilin2/3 and dynamin. It is associated with notch signaling and spine morphology.

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution bycentrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable