SAB4200174

Anti-Rab18 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 同義詞: Anti-RAB18LI1, Anti-RP11-148B2.1, Anti-ras-related protein Rab-18
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: ~23 kDa (additional bands of higher MW may appear in some extract preparations)
• 物種活性: rat, mouse, human
• 包裝: antibody small pack of 25 μL
• 濃度: ~1.0 mg/mL
• 技術: immunoprecipitation (IP): 2.5-5.0 μg using lysates of human PC3 cells; indirect immunofluorescence: 2.5-5.0 μg/mL using rat NRK cells; western blot: 2.5-5.0 μg/mL using whole extracts of mouse MM3MG cells
• UniProt登錄號: Q9NP72
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RAB18(22931) ; mouse ... Rab18(19330) ; rat ... Rab18(307039)

描述

一般說明

Ras-related protein Rab-18 (Rab18) is a member of the ras-associated binding (Rab) family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable C-terminal domains with a cysteine motif, implicated in subcellular targeting. RAB18 gene is mapped to human chromosome 10p12.1.

特異性

Anti-Rab18 (C-terminal) recognizes human, mouse, and rat Rab18.

應用

Anti-Rab18 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

生化／生理作用

Ras-associated binding (Rab)-guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between different subcellular compartments of eukaryotic cells. Their regulatory capacity depends on their ability to cycle between the guanosine diphosphate (GDP)-bound inactive and guanosine triphosphate (GTP)-bound active states. Rab18 is involved in retrograde Golgi-endoplasmic reticulum (ER) trafficking. It localizes to lipid droplets and induces the association of the lipid droplets with endoplasmic reticulum (ER) membranes. Anti-Rab18 may thus be used as a marker to follow the dynamics of lipid droplets in living cells. Rab18 has a potential function in eye development. Mutations in the RAB18 are implicated in Warburg micro syndrome.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

儲存和穩定性

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Discard working dilutions if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable