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SAB4200059

Sigma-Aldrich

Anti-Chimerin β2 antibody, Rat monoclonal

clone HAR 2E3, purified from hybridoma cell culture

同義詞:

Anti-ARHGAP3, Anti-BCH, Anti-CHN2, Anti-Chimaerin β2, Anti-GTPase-activating protein, RHO, 3, Anti-RHOGAP3, Anti-Rho-GTPase-activating protein 3, Monoclonal Anti-Chimerin β2 antibody produced in rat

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rat

共軛

unconjugated

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

HAR 2E3, monoclonal

形狀

buffered aqueous solution

分子量

antigen ~43 kDa

物種活性

human, monkey, rat, mouse, bovine

包裝

antibody small pack of 25 μL

技術

immunocytochemistry: suitable
western blot: 1.0-2.0 μg/mL using Neuro-2a cell extracts

同型

IgG2a

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... CHN2(1124)

一般說明

Monoclonal Anti-Chimerin β2 (rat IgG2a isotype) is derived from the hybridoma HAR 2E3 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a fusion protein of full length human Chimerin β2. Chimerin β2 (CHN2) is a member of the chimerin family and encodes a protein with a phorbol-ester/ diacylglycerol (DAG)-type zinc finger containing C1 domain, a Rho-GTPase activating proteins (GAP) domain and an Src homology 2 (SH2) domain. Chimerin β2 gene is mapped to human chromosome 7p14.3.

特異性

Monoclonal Anti-Chimerin b2 recognizes human, monkey, bovine, mouse and rat Chimerin b2.

免疫原

fusion protein of full length human Chimerin β2

應用

Monoclonal Anti-Chimerin β2 antibody produced in rat has been used in immunoblotting at a dilution of 1:1000 and immunocytochemistry.

生化/生理作用

Chimerin β2 protein (CHN2) is a high affinity receptor for the phorbol esters and diacylglycerol (DAG). Binding of DAG induces translocation of the protein from the cytosol to the Golgi apparatus membrane. It has guanosine triphosphatase GTPase-activating protein activity regulated by phospholipid binding. Decreased expression of CHN2 gene is associated with high-grade gliomas and breast. Deregulation in expression of CHN2 gene is associated with lymphomas, glioblastoma and insulin resistance. Mutations in the CHN2 gene have been associated with schizophrenia in men.

外觀

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

Store at -20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Mitsuhide Maeda et al.
International journal of molecular medicine, 17(4), 559-566 (2006-03-10)
The proliferation and migration of vascular smooth muscle cells (SMC) are important aspects of atherogenesis. Activated growth factor signaling in injured vessels subsequently promotes a number of intracellular events resulting in the phenotypic modulation of SMC. Here, we investigated the
A missense polymorphism (H204R) of a Rho GTPase-activating protein, the chimerin 2 gene, is associated with schizophrenia in men.
Ryota Hashimoto et al.
Schizophrenia research, 73(2-3), 383-385 (2005-01-18)
Victoria Casado-Medrano et al.
Oncotarget, 7(19), 28301-28319 (2016-04-09)
β2-chimaerin is a Rac1-specific negative regulator and a candidate tumor suppressor in breast cancer but its precise function in mammary tumorigenesis in vivo is unknown. Here, we study for the first time the role of β2-chimaerin in breast cancer using
Martin J Baker et al.
Cancers, 12(2) (2020-02-26)
The GTPase Rac1 is a well-established master regulator of cell motility and invasiveness contributing to cancer metastasis. Dysregulation of the Rac1 signaling pathway, resulting in elevated motile and invasive potential, has been reported in multiple cancers. However, there are limited

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