SAB4200017

Anti-MUC1 antibody , Mouse monoclonal

clone 1D1, purified from hybridoma cell culture

• 同義詞: Anti-CD227, Anti-EMA, Anti-H23AG, Anti-KL-6, Anti-MAM6, Anti-Mucin1, Anti-PEM, Anti-PEMT, Anti-PUM
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: 1D1, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 160-500 kDa
• 物種活性: human
• 濃度: ~1.0 mg/mL
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; radioimmunoassay: suitable; western blot: 0.5-1.0 μg/mL using T47D cell lysates
• 同型: IgG1
• UniProt登錄號: P15941
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MUC1(4582)

描述

一般說明

Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. The Mucin 1 (MUC1) gene encodes a membrane bound glycosylated phosphoprotein, belonging to the family of mucins which are heavily glycosylated proteins. Muc1 is a type I transmembrane glycoprotein with a unique extracellular domain containing 20 amino acid variable number tandem repeat (VNTR) domain. In many epithelia, this protein is attached to the apical surface by a transmembrane domain. The degree of glycosylation varies with cell type. The MUC1 gene is mapped to human chromosome 1q22.

免疫原

semi purified MUC1 from pleural effusion of a breast cancer patient.

應用

Monoclonal Anti-MUC1 antibody produced in mouse has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• radioimmunoassay
• immunoprecipitation
• immunocytochemistry

生化／生理作用

Mucin 1 (Muc1) exhibits protective action by binding to pathogens and is also associated with cell signaling capacity. Upregulation of this gene, abnormal intracellular localization and varied glycosylation of Muc1 has been associated with carcinomas. Muc1 might serve as a candidate for single chain antibody mediated targeted therapy in MUC1 expressing cancers. They are preferentially expressed by a variety of adenocarcinomas, including breast, prostate, ovarian and pancreatic carcinomas as well as malignant plasma cells of multiple myelomas.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

法律資訊

Purchase of this product conveys a non-exclusive license to use the product for research purposes only. Purchase of this product does not convey a right to (i) incorporate or use the product in the manufacture of any commercial product, (ii) sell or otherwise transfer the product to any third party, or (iii) use the product to provide any commercial service. THE PRODUCT IS NOT FOR IN VIVO HUMANS USE.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable