SAB3700679

Anti-Rat IgM (μ-chain specific)-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• 同義詞: HRP
• 分類程式碼代碼: 12352203
• NACRES: NA.46

屬性

• 生物源: rabbit
• 共軛: peroxidase conjugate
• 抗體表格: affinity isolated antibody
• 抗體產品種類: secondary antibodies
• 無性繁殖: polyclonal
• 形狀: lyophilized powder
• 物種活性: rat
• 技術: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

Immunoglobulin M (IgM) has a high molecular weight and belongs to the immunoglobulin family. It exists as a pentamer in the serum. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten μ-heavy chains, ten light chains and a single J-chain which are linked by disulphide bonds. The μ-chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as Fragment antigen binding (Fab) fragments. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The heavy chains of IgM contain an extra C domain that replaces the hinge region present between Fab and Fc. IgM is the first antibody to be produced during an immune response and is the major isotype secreted in T-cell independent immune responses.

特異性

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgA or Rat IgG. ELISA was used to confirm specificity at less than 1% cross reactivity against other rat heavy or light chain isotypes.

免疫原

Rat IgM mu heavy chain

物理性質

Antibody format: IgG

外觀

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

重構

Reconstitute with 1.0 mL deionized water (or equivalent).

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P280
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable