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一般說明
Immunoglobulin G (IgG) is a widely expressed serum antibody. It belongs to the immunoglobulin family and consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Immunoglobulin M (IgM) exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten μ-heavy chains, ten light chains and a single J-chain which is linked by disulphide bonds. The μ -chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4. Immunoglobulin A (IgA) is found as two subclasses, that is, IgA1 and IgA2. IgA1 has an extended hinge because of the insertion of a duplicated stretch of amino acids, whereas IgA2 lacks this. IgA exists as a monomer or dimer in the secreted form. Dimeric IgA has a molecular weight of 390kDa. This antibody can form multimers and its polymerization is essential for transport across epithelia. IgA is crucial as the first line of defence and has an important role in mucosal immunity.
特異性
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Fluorescein, Anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM. This reagent is suitable for the detection of all Rat isotypes and chain combinations.
免疫原
Rat IgG, IgA and IgM whole molecules
應用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
物理性質
Antibody format: IgG
外觀
Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
重構
Reconstitute with 1.0 mL deionized water (or equivalent).
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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訊號詞
Danger
危險聲明
危險分類
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2
安全危害
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
The Laboratory Rat (2005)
Structural variation in immunoglobulin constant regions
Immunobiology: The Immune System in Health and Disease (2001)
Structural variation in immunoglobulin constant regions
Immunobiology: The Immune System in Health and Disease (2001)
Suckow MA, et al.
The Laboratory Rat (2005)
Roxana Ramírez-Sandoval et al.
Journal of immunology research, 2015, 269610-269610 (2015-06-13)
Autoimmune nephritis triggered by metallic ions was assessed in a Long-Evans rat model. The parameters evaluated included antinuclear autoantibody production, kidney damage mediated by immune complexes detected by immunofluorescence, and renal function tested by retention of nitrogen waste products and
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