SAB3501192

Anti-TRIM5 delta antibody produced in rabbit

affinity isolated antibody

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: liquid
• 物種活性: human
• 濃度: 1 mg/mL
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_149084
• UniProt登錄號: Q9C035
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TRIM5(85363)

描述

一般說明

TRIM5 delta Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. The TRIM5δ isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5δ also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.

免疫原

TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human TRIM5 delta.The immunogen is located within the last 50 amino acids of TRIM5 delta.

應用

TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 2 μg/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

外觀

Supplied in PBS containing 0.02% sodium azide.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable