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Key Documents

SAB2702380

Sigma-Aldrich

Monoclonal Anti-HEC1 antibody produced in mouse

clone 9G3.23, affinity isolated antibody

同義詞:

HEC, HEC1, HsHec1, KNTC2

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

9G3.23, monoclonal

形狀

buffered aqueous solution

分子量

80kDa

物種活性

hamster, kangaroo rat, human

濃度

1mg/mL

技術

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
proximity ligation assay: suitable
western blot: suitable

同型

IgG2a

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... NDC80(10403)

免疫原

Human HEC1 protein consisting of amino acids 56-642.

應用

Suggested starting dilutions are as follows: FACS: Assay-dependent dilution, ICC/IF: 1:100-1:1000, IHC: Assay-dependent dilution, IP: Assay-dependent dilution, WB: 1:500-1:3000, PLA: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

生化/生理作用

Human HEC 1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results inthe impairment of chromosome congression and results in spindle checkpoint activation.

特點和優勢

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

其他說明

Purification: Protein G Affinity Purified

外觀

Phosphate-buffered saline, no preservative added.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Geethu E Thomas et al.
Nature communications, 7, 11665-11665 (2016-05-27)
Kinetochore couples chromosome movement to dynamic microtubules, a process that is fundamental to mitosis in all eukaryotes but poorly understood. In vertebrates, spindle-kinetochore-associated (Ska1-3) protein complex plays an important role in this process. However, the proteins that stabilize Ska-mediated kinetochore-microtubule

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