S5816

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

• 同義詞: SDF-1α/PBSF
• MDL號碼: MFCD02100631
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 產品名稱: Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse, SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture
• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in E. coli
• 化驗: >97% (SDS-PAGE)
• 形狀: powder
• 效力: 0.15-0.6 ng/mL ED₅₀
• 品質: endotoxin tested
• 分子量: predicted mol wt ~8 kDa
• 包裝: pkg of 10 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: cell culture | mammalian: suitable
• 雜質: <1 EU/μgtested (LAL test)
• UniProt登錄號: P40224
• 儲存溫度: −20°C
• 基因資訊: mouse ... Cxcl12(20315)

描述

一般說明

Stromal derived factor-1 (SDF-1) is localized to adipocytes.

應用

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.

生化／生理作用

SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..

Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.

外觀

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

準備報告

Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.

分析報告

The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)