S304-05A

Human Aortic Endothelial Cells: HAOEC: Pre-Screened for Angiogenesis & VEGF signaling, adult

• 分類程式碼代碼: 41106514
• NACRES: NA.81

屬性

• 生物源: human blood vessels
• 品質等級: 100
• 包裝: pkg of 500,000 cells
• 製造商／商標名: Cell Applications, Inc
• 增長模式: Adherent
• 染色體組型: 2n = 46
• 形態學: Endothelial
• 技術: cell culture | mammalian: suitable
• 相關疾病: diabetes; cardiovascular diseases
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

一般說明

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Endothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development.

Matched smooth muscle cells obtained from the same arteries are available as well.The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.

VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.

Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells express the following important biomarkers:

• Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes;
• eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
• Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
• Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.

細胞系來源

Blood Vessels

應用

cardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testing

成分

Basal Medium containing 10% FBS & 10% DMSO

準備報告

• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

例行更新培養

Please refer to the HAOEC Culture Protocol.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable