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Key Documents

S0698

Sigma-Aldrich

Anti-SmB antibody, Mouse Monoclonal

clone 12F5, purified from hybridoma cell culture

同義詞:

Anti-SNRPB1, Anti-Sm protein B, Anti-Sm-B, Anti-SmB/SmB, Anti-Small nuclear ribonucleoprotein associated protein B, Anti-snRNP-B

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

12F5, monoclonal

分子量

antigen ~25 kDa

物種活性

human, mouse, canine

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear cell extract

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... SNRPB(6628)
mouse ... Snrpb(20638)

一般說明

Monoclonal Anti-SmB (mouse IgG1 isotype) is derived from the hybridoma 12F5-H3.D8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an SmB recombinant protein.
SNRPB (small nuclear ribonucleoprotein-associated protein B) is an important constituent of the spliceosome. It codes for SmB/B′ (small nuclear ribonucleoprotein polypeptides B and B1). It is located on human chromosome 20p13. SmB′/B are upregulated in all tissues excluding postnatal brain.

免疫原

SmB recombinant protein.

應用

  • Monoclonal Anti-SmB antibody produced in mouse has been used in:
  • western blotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • immunocytochemistry

生化/生理作用

SNRPB (small nuclear ribonucleoprotein-associated protein B) mutations results in cerebro-costo-mandibular syndrome. Downregulation of SNRPB affects the upregulation and processing of RNA splicing machinery components. It also plays a major role in RNA stability.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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存取文件庫

SmD3 regulates intronic noncoding RNA biogenesis
Scruggs BS, et al.
Molecular and Cellular Biology, 32(20), 4092-4103 (2012)
CARM1 is essential for myeloid leukemogenesis but dispensable for normal hematopoiesis
Greenblatt SM, et al.
Cancer Cell, 33, 1111-1127 (2018)
Michael Briese et al.
Nature structural & molecular biology, 26(10), 930-940 (2019-10-02)
Studies of spliceosomal interactions are challenging due to their dynamic nature. Here we used spliceosome iCLIP, which immunoprecipitates SmB along with small nuclear ribonucleoprotein particles and auxiliary RNA binding proteins, to map spliceosome engagement with pre-messenger RNAs in human cell
Functional genomics analyses of RNA-binding proteins reveal the splicing regulator SNRPB as an oncogenic candidate in glioblastoma
Correa BR, et al.
Genome Biology, 17(1) (2016)
U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein
Skrajna A, et al.
RNA, 23(6), 938-951 (2017)

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