推薦產品
一般說明
麦胚 tRNA 含有许多不同的特异性 tRNA 分子。
應用
粗转移 RNA (tRNA) 从小麦胚芽中提纯,含有许多特定的 tRNA 分子,可以通过不同的过程分离。利用 体外 翻译系统进行详细的结构和功能研究实验通常需要高纯度的 tRNA,这可以通过色谱分离和 tRNA 的可逆生化修饰的组合来实现。麦胚 tRNA 已用于从麦胚纯化 RNAse Z(tRNA 3′-处理核酸内切酶)。纯化包括 6 个步骤,其中最有效的步骤是使用由麦胚 tRNA 构成的亲和柱,其与 RNAse Z 紧密结合。
适合作为核酸纯化和沉淀中的载体使用。
成分
tRNA 以冻干粉形式提供,可溶于水,浓度为 2 mg/mL。
單位定義
在 1.0 mL 水中(1 cm 光径),1 个单位在 260 nm 处的吸光度为 1.0。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening.
Methods in cell biology, 77, 505-519 (2004-12-18)
Nature protocols, 3(1), 59-69 (2008-01-15)
The in situ hybridization (ISH) technique allows the sites of expression of particular genes to be detected. This protocol describes ISH of digoxigenin-labeled antisense RNA probes to whole-mount zebrafish embryos. In our method, PCR-amplified sequence of a gene of interest
Nature genetics, 30(3), 329-334 (2002-02-19)
Post-translational modification of histone tails is thought to modulate higher-order chromatin structure. Combinations of modifications including acetylation, phosphorylation and methylation have been proposed to provide marks recognized by specific proteins. This is exemplified, in both mammalian cells and fission yeast
PLoS genetics, 15(1), e1007730-e1007730 (2019-01-18)
Meiosis is a cellular program that generates haploid gametes for sexual reproduction. While chromosome events that contribute to reducing ploidy (homologous chromosome pairing, synapsis, and recombination) are well conserved, their execution varies across species and even between sexes of the
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9716-9721 (2013-05-01)
After four decades of research aimed at understanding tRNA selection on the ribosome, the mechanism by which ribosomal ambiguity (ram) mutations promote miscoding remains unclear. Here, we present two X-ray crystal structures of the Thermus thermophilus 70S ribosome containing 16S
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