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Key Documents

R7638

Sigma-Aldrich

RPMI-1640 培养基

Dutch Modification, with sodium bicarbonate and HEPES, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

同義詞:

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

RPMI-1640 培养基, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

pH值

>7.2

成分

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。

應用

RPMI-1640 Medium has been used

  • to obtain spleen and lymph nodes from mice
  • for the resuspension of Staphylococcus aureus strains in the phagocytosis assay
  • in the isolation of hepatitis B surface antigen-specific B-cell clones

重構

Supplement with 0.3 g/L L-glutamine.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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