R3779

Anti-RbAp48/RbAp46 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 同義詞: Anti-Retinoblastoma-binding protein
• MDL號碼: MFCD02686588
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: IgG fraction of antiserum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 46 kDa (RbAp46); antigen 48 kDa (RbAp48)
• 物種活性: human
• 技術: indirect immunofluorescence: 1:500-1:1,000 using human HeLa cells fixed with paraformaldehyde-Triton; microarray: suitable; western blot: 1:2,000-1:4,000 using MCF7 nuclear extracts
• UniProt登錄號: Q16576
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RBBP4(5928) , RBBP7(5931) ; mouse ... Rbbp4(19646) , Rbbp7(245688) ; rat ... Rbbp4(313048) , Rbbp7(83712)

描述

一般說明

pRB-associated proteins p48 (RbAp48) is a 425 amino acids WD-domain protein isolated as a RB1 (retinoblastoma binding protein). RbAp46 (also known as RBBP7- retinoblastoma-binding protein 7) has 89% amino acid identity with RbAp48.

免疫原

synthetic peptide corresponding to amino acids 413-425 of human RbAp48, conjugated to KLH via an N-terminal added lysine residue. The immunizing peptide is conserved in human, mouse, and rat.

應用

Anti-RbAp48/RbAp46 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

生化／生理作用

RbAp46 and RbAp48 are two histone tail-targeting proteins, that are found in association with chromatin remodeling complexes Sin3A/ histone deacetylase (HDAC) and NURD (nucleosomal remodeling and deacetylation complex). Transcriptional repressors exert their effects by recruitment of the Sin3A/HDAC corepressor complex, which contains a module composed of Sin3A, HDAC1, HDAC2, RbAp46, RbAp48, SAP30, and others. In the NuRD complex, HDAC1, HDAC2, RbAp48, and RbAp46 associate with MTA2 (metastasis associated 1 family member 2), MBD3 (methyl-CpG binding domain protein), MAT1L1, MBD3L1 (methyl-CpG binding domain protein 3 like 1), CHD3, and CHD4 to form the nucleosomal remodeling and deacetylation (NuRD) complex.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)