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Key Documents

R2655

Sigma-Aldrich

抗兔IgG,天然抗体,小鼠单克隆 小鼠抗

clone RabT-50, purified from hybridoma cell culture

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

secondary antibodies

無性繁殖

RabT-50, monoclonal

形狀

buffered aqueous solution

物種活性

rabbit

應無反應活性

human, rat, chicken, cat, bovine, monkey, goat, pig, horse

濃度

~1 mg/mL

技術

immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane

同型

IgG1

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

相關類別

一般說明

抗兔IgG天然抗体,小鼠单克隆抗体(小鼠IgG1同种型)来源于小鼠骨髓瘤细胞(NS1)和BALB/c小鼠脾细胞融合RabT-50杂交瘤。人血清中大量存在免疫球蛋白G(IgG)。它约占血浆蛋白的10-20%。IgG由糖蛋白组成,其中有82-96%的蛋白质和4-18%的碳水化合物。它由四个亚类组成,即IgG1,IgG2,IgG3和IgG4。IgG由四条多肽链组成-两条重链(γ链)和两条轻链(κ或λ链),它们通过链间二硫键连接。重链和轻链在VH和CH1结构域连接,形成片段抗原结合(Fab)臂。抗原与抗体的V区结合。
该抗体抗与非还原型兔IgG发生特异性反应,不与还原型兔IgG发生反应。

特異性

抗兔IgG,天然抗体,小鼠单克隆抗体与非还原型兔IgG发生特异性反应,不与还原型兔IgG发生反应。它不能识别人,猴,牛,马,猪,山羊,猫,大鼠和鸡的IgG。

免疫原

兔IgG轻链的富集部分。

應用

兔抗IgG天然抗体,小鼠单克隆抗体已用于:
  • 免疫印迹
  • 免疫细胞化学
  • 免疫组织化学
  • 酶链吸附测定(ELISA)

生化/生理作用

IgG抗体亚型是免疫系统中最丰富的血清免疫球蛋白。它由B细胞分泌,存在于血液和细胞外液中,可防止细菌、真菌和病毒引起的感染。母体IgG可通过胎盘转移至胎儿,胎盘对新生儿免疫防御感染至关重要。

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

儲存和穩定性

若需延长储存时间,可将溶液分装并在-20℃下冷冻。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存时出现轻微浑浊,请在使用前通过离心澄清溶液。若工作稀释样品在12小时内未使用完,则应丢弃。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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