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P9620

Sigma-Aldrich

嘌呤霉素

Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, suitable for cell culture

同義詞:

3′-(L-α-Amino-p-methoxyhydrocinnamamido)-3′-deoxy-N,N-dimethyladenosine dihydrochloride, Stylomycin dihydrochloride

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About This Item

CAS號碼:
58-58-2
MDL號碼:
MFCD00012691
分類程式碼代碼:
51281906
PubChem物質ID:
329820352
NACRES:
NA.85

生物源

Streptomyces alboniger

品質等級

300

種類

for cell culture

化驗

>98% (HPLC)

形狀

solution

儲存條件

(Store in cool place. Keep container tightly closed in a dry and well -ventilated place. )

濃度

10 mg/mL in H2O

技術

cell culture | mammalian: suitable

顏色

colorless to yellow

溶解度

H2O: soluble 10 mg/mL

抗生素活性譜

Gram-positive bacteria
neoplastics
parasites

作用方式

protein synthesis | interferes

運輸包裝

wet ice

儲存溫度

−20°C

SMILES 字串

Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

InChI 密鑰

MKSVFGKWZLUTTO-FZFAUISWSA-N

一般說明

嘌呤霉素，也称杆菌肽或嘌啉霉素盐酸盐，是一种氨基核酸酶抗生素和蛋白质合成抑制剂。它是由土壤放线菌白黑链霉菌（Streptomyces alboniger）通过一系列酶促反应合成。嘌呤霉素有效抑制原核和真核细胞，其作用原理是干扰RNA功能，从而抑制蛋白质合成。它也充当氨基肽酶和脑啡肽酶的抑制剂。嘌呤霉素可以使细菌、原生动物、水藻和哺乳动物细胞的生长迅速停止，但是含pac基因的细胞有耐药性。 pac基因的基因产物是嘌呤霉素-N-乙酰基转移酶，这种酶通过乙酰化反应使嘌呤霉素失活。

嘌呤霉素与tRNA的含氨基末端结构相似，这一特性令其在蛋白质合成过程中进入核糖体，结合新生多肽链，并使链的延长停止。嘌呤霉素广泛用于细胞培养，可作为以耐嘌呤霉素基因转化的细胞的选择剂。

應用

嘌呤霉素是一种氨基核苷酸抗生素，衍生自白黑链霉菌。 它可以选择包含抗性基因嘌呤霉素 N-乙酰基转移酶（PAC）的细胞。 它被用在兔模型中，研究处理后的血管平滑肌细胞的生存能力。 嘌呤霉素用在体细胞克隆和胚胎移植后产生增强的绿色荧光蛋白（EGFP）转基因仔猪。

生化／生理作用

作用机制：嘌呤霉素通过提前终止链来抑制蛋白质合成，作为氨基酰基-tRNA 的 3′ 末端的类似物起作用。嘌呤霉素还可作为二肽基肽酶 II（丝氨酸肽酶）和胞质溶胶丙氨酰氨基肽酶的可逆抑制剂起作用。

耐药性机制：嘌呤霉素乙酰转移酶是有效的抗性基因。

抗菌谱：该产品对革兰氏阳性微生物有活性，对耐酸杆菌的活性较低，对革兰氏阴性微生物的活性更低。 嘌呤霉素可以防止细菌、原生动物、藻类和哺乳动物细胞的生长，并且起效迅速，可在 2 天内杀死 99％ 的细胞。

特點和優勢

  • 适用于多种研究应用的优质抗生素
  • 抗生素和蛋白质合成抑制剂
  • 有效抑制原核和真核细胞
  • 通过干扰RNA功能抑制蛋白质合成
  • 常用作细胞生物学研究中的选择剂

準備報告

将该产物以 10 mg/mL 的浓度浓缩在水中。已在转染了 pac 耐药基因的 HeLa 细胞上测试了其生长抑制和细胞选择的能力。

其他說明

保存于密闭容器内，置于干燥通风处。
如需了解生化试剂系列的更多信息，请填写此表。

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產品號碼
描述
訂價

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Satoshi Watanabe et al.
Biology of reproduction, 72(2), 309-315 (2004-09-24)
Puromycin N-acetyl transferase gene (pac), of which the gene product catalyzes antibiotic puromycin (an effective inhibitor of protein synthesis), has been widely used as a dominant selection marker in embryonic stem (ES) cell-mediated transgenesis. The present study is the first
Jacinta P W Lee et al.
Autophagy, 12(6), 907-916 (2016-05-11)
MIF (macrophage migration inhibitory factor [glycosylation-inhibiting factor]) is a pro-inflammatory cytokine expressed in multiple cells types, including macrophages. MIF plays a pathogenic role in a number of inflammatory diseases and has been linked to tumor progression in some cancers. Previous
Ming Lee Lin et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(8), 3029-3034 (2008-02-15)
Cross-presentation as a fundamental pathway of activating CD8(+) T cells has been well established. So far the application of this concept in vivo is limited, and the mechanisms that specialize CD8(+) dendritic cells (DCs) for this task are not fully
S Eyckerman et al.
Nature cell biology, 3(12), 1114-1119 (2002-01-10)
Ligand-induced clustering of type I cytokine receptor subunits leads to trans-phosphorylation and activation of associated cytosolic janus kinases (JAKs). In turn, JAKs phosphorylate tyrosine residues in the receptor tails, leading to recruitment and activation of signalling molecules. Among these, signal
Valerie Croons et al.
The Journal of pharmacology and experimental therapeutics, 325(3), 824-832 (2008-03-07)
Recent evidence indicates that the protein synthesis inhibitor cycloheximide triggers selective macrophage death in rabbit atheroma-like lesions without affecting smooth muscle cells (SMCs) or the endothelium, thereby favoring a stable plaque phenotype. In this study, we report that puromycin, a
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