推薦產品
化驗
≥99% (titration)
形狀
crystalline powder
pH值
4.5-4.7
有用的pH值範圍
6.1-7.5
pKa (25 °C)
6.8
溶解度
0.1 M NaOH: 250 mg/mL, clear, colorless
應用
diagnostic assay manufacturing
儲存溫度
room temp
SMILES 字串
[Na+].OS(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O6S2.Na/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;/h1-8H2,(H,11,12,13)(H,14,15,16);/q;+1/p-1
InChI 密鑰
OGGAIRCLBMGXCZ-UHFFFAOYSA-M
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一般說明
1, 4-哌嗪二乙磺酸(PIPES)是一种两性离子缓冲液,属于乙磺酸缓冲液系列,由Good等人率先推出,是一种用于细胞培养、电子显微镜组和其他生物学应用织固定的无毒缓冲液。PIPES 不与金属离子相互作用。由于其在37°C时的pKa接近生理pH,因此在生物、生化和环境研究中被用作缓冲物质。PIPES满足特定的标准:中值pKa,最大水溶性且在所有其他溶剂中的溶解度极小,盐效应极低,pKa随温度变化极小,化学和酶学稳定,在可见光或紫外光谱范围吸光度极小,易于合成。可将碱溶液添加到不含 PIPES 的酸中并滴定至适当的 pH 值,或通过混合单钠盐和二钠盐的等摩尔溶液并滴定至适当的 pH 值,制备缓冲液。
應用
PIPES 钠盐缓冲液可用于:
- 配制 BRB80 缓冲液,以通过荧光显微镜对动态微管和单个驱动蛋白分子成像
- 作为 PEM 固定液成分
- 复溶红细胞 (RBC),以研究 pH 对 5 配位α-亚硝酰血红蛋白 (HbNO) 稳定性的影响
特點和優勢
- 无毒
- 易用
- 热稳定
- 易于处理和储存
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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