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Key Documents

P1034

Sigma-Aldrich

异硫氰酸苯酯

Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid

同義詞:

PITC

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About This Item

線性公式:
C6H5NCS
CAS號碼:
分子量::
135.19
Beilstein:
471392
EC號碼:
MDL號碼:
分類程式碼代碼:
12352118
PubChem物質ID:
NACRES:
NA.31

等級

Sigma Grade

品質等級

化驗

≥99% (GC)

形狀

liquid

濃度

8.36 M

折射率

n20/D 1.6515 (lit.)

bp

218 °C (lit.)

mp

−21 °C (lit.)

密度

1.132 g/mL at 20 °C (lit.)

適合性

suitable for solid phase protein sequencing analysis

儲存溫度

2-8°C

SMILES 字串

S=C=Nc1ccccc1

InChI

1S/C7H5NS/c9-6-8-7-4-2-1-3-5-7/h1-5H

InChI 密鑰

QKFJKGMPGYROCL-UHFFFAOYSA-N

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一般說明

异硫氰酸苯酯属于带异硫氰酸基团的芳香族化合物类。它通常用作柱前衍生剂,用于形成苯硫代氨基甲酰基氨基酸,以便通过色谱技术进行测定。

應用

异硫氰酸苯酯可用于通过自动埃德曼反应和之后的高效液相色谱分析(HPLC)技术,在亚皮摩尔和亚纳摩尔水平进行多肽和蛋白质测序。
用于伯胺和仲胺的衍生化试剂。用于通过 Edman 降解法进行多肽测序以及通过 HPLC 进行氨基酸分析。

包裝

氩气下用火焰封装的 1mL 棕色安瓿瓶包装。
氩气下装瓶的 10mL 螺纹盖棕色样品瓶包装。

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

190.4 °F - closed cup

閃點(°C)

88 °C - closed cup

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


分析證明 (COA)

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

R F Ebert
Analytical biochemistry, 154(2), 431-435 (1986-05-01)
A method for analysis of phenylthiocarbamyl-amino acids by HPLC is described. Solvent A contains 0.05 M sodium acetate plus triethylamine (2.75 ml/liter) adjusted to pH 6.40 with phosphoric acid. Solvent B consists of 50% solvent A, 40% acetonitrile, and 10%
R L Heinrikson et al.
Analytical biochemistry, 136(1), 65-74 (1984-01-01)
Methods for the quantitative derivatization of amino acids with phenylisothiocyanate and for the separation and quantitation of the resulting phenylthiocarbamyl derivatives by reverse-phase high-performance liquid chromatography are described. Phenylthiocarbamylation of amino acids proceeds smoothly in 5 to 10 min at
Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis
Tempst P and Riviere L
Analytical Biochemistry, 183(2), 290-300 (1989)
Misako Aito-Inoue et al.
Journal of agricultural and food chemistry, 54(15), 5261-5266 (2006-07-20)
For the isolation and detection of food-derived peptides in blood, an approach based on the derivatization of peptides with phenyl isothiocyanate (PITC) was developed. This approach allows hydrophilic peptides to be resolved and specifically detected by reversed-phase (RP) HPLC. For
R Gheshlaghi et al.
Analytical biochemistry, 383(1), 93-102 (2008-08-30)
Modified resolution and overall separation factors used to quantify the separation of complex chromatography systems are described. These factors were proven to be applicable to the optimization of amino acid resolution in reverse-phase (RP) HPLC chromatograms. To optimize precolumn derivatization

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