P0117

Monoclonal Anti-PIAS3 小鼠抗

~2 mg/mL, clone PIA3, purified immunoglobulin, buffered aqueous solution

• 同義詞: Anti-Protein Inhibitor of Activated STAT 3
• MDL號碼: MFCD09265430
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: PIA3, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~68 kDa (additional band at 44 kDa may be present)
• 物種活性: chicken, mouse, canine, human, bovine
• 濃度: ~2 mg/mL
• 技術: immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 2-4 μg/mL using HepG2 total cell extract
• 同型: IgG1
• UniProt登錄號: Q9Y6X2
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PIAS3(10401) ; mouse ... Pias3(229615)

描述

一般說明

Protein inhibitor of activated STAT protein 3 (PIAS3) is an inhibitor of activated STAT3. The gene encodes for a 619-amino acid protein and has a molecular mass of 68 kDa. PIAS3 shares 55% identity with PIAS1 and PIASX proteins and 39% identity with PIASY. PIAS3 is widely expressed in various human tissues.

免疫原

synthetic peptide corresponding to amino acids 591-604 of human PIAS3.

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Monoclonal Anti-PIAS3 antibody produced in mouse has been used in:

• enzyme linked immuno sorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

生化／生理作用

Protein inhibitor of activated STAT protein 3 (PIAS3) and other members of this family can act as small ubiquitin-like modifier (SUMO) E3 ligases and interfere with the transcription activity of many transcription factors. PIAS3 binds to microphthalmia-associated transcription factor (MITF), a key DNA-binding protein. It blocks MITF DNA-binding activity and co-expression of MITF. PIAS3 in NIH 3T3 cells inhibits MITF-driven transcription activity.

標靶描述

PIAS3 encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)