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N2913

Sigma-Aldrich

N-TER™ Nanoparticle siRNA Transfection System

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.51

形狀

liquid

運輸包裝

wet ice

儲存溫度

−20°C

應用

N-TER纳米粒siRNA转染系统为基于肽的转染系统，可用于真核细胞的瞬时基因敲除。N-TER肽可以与siRNA非共价结合，形成纳米颗粒。这种纳米颗粒可以直接与脂质膜表面上的脂质体相互作用，从而使纳米颗粒分散到细胞膜上，进而将siRNA直接递送到细胞质中。和大多数基于脂质的转染方法不同，这种新的递送机制规避了内涵体通路，从而大大降低了siRNA在转染过程中被降解的可能性。

法律資訊

在CNRS许可下，由Sigma-Aldrich生产并分销。
N-TER is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Federica Simeoni et al.
Nucleic acids research, 31(11), 2717-2724 (2003-05-29)
The improvement of non-viral-based gene delivery systems is of prime importance for the future of gene and antisense therapies. We have previously described a peptide-based gene delivery system, MPG, derived from the fusion peptide domain of HIV-1 gp41 protein and
L Crombez et al.
Biochemical Society transactions, 35(Pt 1), 44-46 (2007-01-20)
The major obstacle to clinical development of siRNAs (short interfering RNAs), like for most of the nucleic-acid-based strategies, is their poor cellular uptake and bioavailability. Although several viral and non-viral strategies have been proposed to improve siRNA delivery, their applications
Sébastien Deshayes et al.
Advanced drug delivery reviews, 60(4-5), 537-547 (2007-11-27)
The recent discovery of new potent therapeutic molecules which do not reach the clinic due to poor delivery and low bioavailability have made of delivery a key stone in therapeutic development. Several technologies have been designed to improve cellular uptake
Linlu Tian et al.
Scientific reports, 5, 12357-12357 (2015-07-24)
Melanoma is one of the most aggressive skin cancers and is well known for its high metastatic rate. Studies have shown that epithelial mesenchymal transition (EMT) is essential for melanoma cell metastasis. However, the molecular mechanisms underlying EMT are still
Joshua E Lewis et al.
Antioxidants & redox signaling, 29(10), 937-952 (2017-08-02)
The purpose of this study was to investigate differential nicotinamide adenine dinucleotide phosphate, reduced (NADPH) production between radiation-sensitive and -resistant head and neck squamous cell carcinoma (HNSCC) cell lines and whether these differences are predictive of sensitivity to the chemotherapeutic
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文章

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

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