MTOXCE2E1

CypExpress^™ 2E1 Cytochrome P450 human

• 同義詞: CYP2E, CypP450 2E1, CPE1, P450-J, P450C2E, cytochrome P450 family 2 subfamily E member 1
• 分類程式碼代碼: 12352200
• NACRES: NA.77

屬性

• 生物源: human
• 重組細胞: expressed in Pichia pastoris
• 形狀: dry powder
• 儲存溫度: −70°C
• 基因資訊: human ... CYP2E1(1571)

描述

生化／生理作用

Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. The gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous.

特點和優勢

CypExpress^™ 2E1 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP 2E1 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress^™ are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress^™ retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems.It is designed for rapid screening of drug metabolites and for scaling up metabolite generation.

法律資訊

CypExpress is a trademark of Oxford Biomedical Research

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable