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一般說明
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG AHR knockout cells express the major xenobiotic sensors (PXR and CAR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
應用
See technical bulletin for detailed protocols
特點和優勢
HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity. - Sigma′s HepaRG AHR Knockout (KO) allows conclusive identification of CYP1A1 and CYP1B1 induction through AHR activation.
Zinc finger nucleases (ZFN) mediated Knockout of AHR (AHR) gene.
- The frame-shift mutation of AHR gene was confirmed by fragment length analysis and DNA sequencing.
- Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.
品質
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
法律資訊
Exhibit 1: ADME/Tox cell lines license
Exhibit 2: HepaRG limited use license
Exhibit 2: HepaRG limited use license
HepaRG is a trademark of BioPredic International company
免責聲明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
E F O'Donnell et al.
Cell death & disease, 5, e1038-e1038 (2014-02-01)
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Ziyu Liu et al.
Journal of molecular histology, 44(4), 455-461 (2013-04-03)
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor implicated in multiple cellular processes and its expression has been shown to play a critical role in tumorigenesis. However, the role of AhR in tumorigenesis of hepatocellular carcinoma remains unclear.
Dániel Szöllősi et al.
PloS one, 11(1), e0146066-e0146066 (2016-01-05)
Understanding of multidrug binding at the atomic level would facilitate drug design and strategies to modulate drug metabolism, including drug transport, oxidation, and conjugation. Therefore we explored the mechanism of promiscuous binding of small molecules by studying the ligand binding
Marc Le Vee et al.
Toxicology in vitro : an international journal published in association with BIBRA, 24(6), 1775-1781 (2010-07-14)
Aryl hydrocarbon receptor (AhR) is a drug-sensing receptor activated by environmental contaminants such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and is known to drive regulation of target genes in various human cell types. Its involvement in TCDD-mediated regulation of target genes in human
Hong Feng Zhang et al.
Alcoholism, clinical and experimental research, 36(11), 1873-1881 (2012-04-11)
During the course of alcohol-induced liver damage, hepatic stellate cells are transformed into proliferative, fibrogenic, and contractile myofibroblasts. Aryl hydrocarbon receptor (AhR) is a transcription factor that controls the expression of genes involved in the metabolism of xenobiotics, inflammation, cell
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