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MP0035

Sigma-Aldrich

LookOut ® 支原体 PCR 检测试剂盒

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

同義詞:

PCR-based mycoplasma screening

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質

Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum

品質等級

100

包裝

pkg of 1 kit

技術

PCR: suitable

應用

detection
microbiology

相容性

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

儲存溫度

2-8°C

一般說明

该LookOut®支原体PCR检测试剂盒利用聚合酶链反应（PCR），这是因其在对细胞培养基中的支原体、无胆甾原体和尿素原体以及其他细胞培养衍生的生物制品的检测中具有最高灵敏度而被确定作为选择的方法。该试剂盒已被成功测试具有对20,000至20个拷贝的发酵支原体基因组DNA进行扩增的能力。
包含在试剂盒内的反应管预涂覆有适当的dNTP、引物和上样染料。 相比于需要对反应管进行单独上样的一般方法，整体的检测时间得到了大幅度的下降。

應用

支原体PCR检测试剂盒视频实验方法
LookOut®支原体PCR检测试剂盒已用于支原体感染筛查。

相容性

该试剂盒已被优化与货号D9307一起使用。不推荐任何带有3′-5′外切核酸酶活性的DNA聚合酶。这些包括Tli、Pfu和Pwo。那些可被使用的DNA聚合酶包括Taq、Tfl和Tth。

法律資訊

JumpStart is a trademark of Sigma-Aldrich Co. LLC
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

產品號碼
描述
訂價

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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文章

Mycoplasma Detection and Elimination

Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.

條款

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

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