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Merck
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Key Documents

MAK552

Sigma-Aldrich

Fluo-4 No Wash Calcium Assay Kit

sufficient for fluorometric test plates

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About This Item

分類程式碼代碼:
41116104
NACRES:
NA.84

檢測方法

fluorometric

相關疾病

endocrinological disorders, diabetes; genitourinary disorders; neurological disorders; cancer

儲存溫度

−20°C

一般說明

The Fluo-4 No Wash Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are preloaded with Fluo-4 AM which can cross the cell membrane. Once inside the cell, the lipophilic blocking groups of Fluo-4 AM are cleaved by non-specific cell esterase, resulting in a negatively charged Fluo-4 dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells are stimulated with screening compounds, the receptor signals the release of intracellular calcium, which greatly increases the fluorescence of Fluo-4.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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