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Merck
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重要文件

MAK518

Sigma-Aldrich

ADP Assay Kit

sufficient for 100 fluorometric tests

同義詞:

ADP Detection Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

檢測方法

fluorometric

相關疾病

hematological disorder

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Adenosine diphosphate (ADP) is the product of ATP dephosphorylation by ATPases. ADP can be converted back to ATP by ATP synthases. ADP levels regulate several enzymes involved in intermediary metabolism. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, since these assays require measurement of ATP in the sample before conversion of ADP to ATP, if the nascent ATP concentration is significantly higher than the ADP concentration, the ATP signal will drown out the ADP signal.

特點和優勢

  • As low as 0.1 µM ADP can be quantified.
  • Compatible with high-throughput handling systems.

適合性

The kit is suitable for ADP determination in cells and other biological samples.

原則

The ADP Assay Kit provides a convenient fluorometric means to measure ADP level even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate is then quantified by a fluorometric method at λEx = 530 nm/λEm = 590 nm.

訊號詞

Danger

危險分類

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 2


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分析證明 (COA)

Lot/Batch Number

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