MAK493

Isocitrate Dehydrogenase (IDH) Assay Kit

sufficient for 100 colorimetric tests

• 分類程式碼代碼: 12352204
• NACRES: NA.84

屬性

• 品質等級: 100
• 檢測方法: colorimetric
• 相關疾病: hematological disorder; cancer
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

Isocitrate dehydrogenase (IDH) is an enzyme which catalyzes the interconversion of isocitrate and α-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD⁺ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP⁺ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the IDH1 and IDH2 isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia.

特點和優勢

• Linear detection range 0.1 to 100 U/L for 30 minute reaction.
• Compatible with high-throughput handling systems.

適合性

The kit is suitable for IDH activity determination in biological samples (e.g. plasma, serum, tissue and culture media.)

原則

The Isocitrate Dehydrogenase (IDH) Assay Kit is a non-radioactive, colorimetric IDH assay based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT, which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

安全資訊

• 象形圖: GHS02,GHS05,GHS07
• 訊號詞: Danger
• 危險聲明: H225,H290,H315,H319,H336
• 防範說明: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• 危險分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• 標靶器官: Respiratory system
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 2