MAK380

LDH-Cytotoxicity Assay Kit II

sufficient for 500 colorimetric tests

• 分類程式碼代碼: 12161503

屬性

• 檢測方法: colorimetric
• 儲存溫度: −20°C

描述

一般說明

The new LDH Cytotoxicity Assay Kit, MAK529, is now available! Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydrogenase (LDH) is a stable enzyme, present in all cell types, and rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

特點和優勢

The LDH-Cytotoxicity Assay Kit II:

• Is a non-radioactive assay.
• Simple one-step procedure.

適合性

The kit is suitable for the determination of Lactate dehydrogenase (LDH) activity in cells.

原則

The LDH Cytotoxicity Assay Kit II utilizes the advanced WST (Water Soluble Tetrazolium) reagent for a fast and more sensitive detection of LDH released from the damaged cells. The assay utilizes an enzymatic coupling reaction: LDH oxidizes lactate to generate NADH, which then reacts with WST to generate yellow color at A450 nm. The intensity of the generated color correlates directly with the cell number lysed.

安全資訊

• 象形圖: GHS05
• 訊號詞: Danger
• 危險聲明: H314
• 防範說明: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• 危險分類: Eye Dam. 1 - Skin Corr. 1B
• 儲存類別代碼: 8A - Combustible corrosive hazardous materials