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Merck
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Key Documents

M7403

Sigma-Aldrich

MCDB 153培养基

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

同義詞:

Molecular, Cellular, and Development Biology 153 medium

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

MCDB 153培养基是对Ham营养混合物F-12的改良,设计用于人角质形成细胞的生长、鸡胚成纤维细胞的克隆生长和中国仓鼠卵巢(CHO)细胞的生长,使用低水平的胎牛血清蛋白(FBSP)、大量微量元素或无血清蛋白。

應用

MCDB 153培养基已用作黑色素瘤分离培养基(MIM)的组分,用于培养人类非转移性和转移性黑色素瘤细胞。

數量

配方中每升培养基含有17.6克的粉末。

重構

并补充1.18 g/L碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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