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M1919

Sigma-Aldrich

Monoclonal Anti-Macrophages antibody produced in mouse

clone LN-5, ascites fluid

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

共軛

unconjugated

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

LN-5, monoclonal

包含

15 mM sodium azide

物種活性

human

技術

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using formalin-fixed, paraffin-embedded human tonsil sections

同型

IgM

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

相關類別

一般說明

Monoclonal Anti-Human Macrophages (mouse IgM isotype) is derived from the LN-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Macrophages are widely distributed throughout the body. They belong to the mononuclear phagocytes family. Macrophages have abundant cytoplasm and are also termed histiocyte.

免疫原

deparaffinized human peripheral blood mononuclear cells

生化/生理作用

Macrophages play a crucial role in body defense mechanisms and have phagocytic functionality. They are heterogeneous in morphology, cytochemistry, function and surface antigens. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and the diagnosis of various inflammation.

外觀

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Slava Epelman et al.
Immunity, 41(1), 21-35 (2014-07-19)
Macrophages are distributed in tissues throughout the body and contribute to both homeostasis and disease. Recently, it has become evident that most adult tissue macrophages originate during embryonic development and not from circulating monocytes. Each tissue has its own composition
L Bhoopat et al.
Blood, 71(4), 1079-1085 (1988-04-01)
Two monoclonal antibodies (LN-4, LN-5) reactive to human macrophages in B5 formalin-fixed, paraffin-embedded tissue sections have been produced by using deparaffinized cell extracts of peripheral blood mononuclear cells. Both monoclonal antibodies were initially identified on paraffin-embedded sections of hyperplastic lymph
S S Shin et al.
Human pathology, 23(6), 686-694 (1992-06-01)
To provide baseline information on the immunoarchitecture of normal bone marrow, we studied cryostat-cut, frozen, and paraffin-embedded, fixed tissue sections prepared from 21 core biopsies of normal bone marrow obtained during bone marrow harvests for transplantation. A large panel of

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