M1445

Anti-Mus81 antibody, Mouse monoclonal

clone MTA30 2G10/3, purified from hybridoma cell culture

• 同義詞: Anti-SLX3
• MDL號碼: MFCD08705592
• 分類程式碼代碼: 12352203
• NACRES: NA.41
• 共軛: unconjugated
• application: IP; WB
• 無性繁殖: MTA30 2G10/3, monoclonal
• 物種活性: human, monkey
• citations: 13
• 技術: immunoprecipitation (IP): suitable; western blot: 0.5-1 μg/mL using HEK-293T total cell extract

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: MTA30 2G10/3, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~59 kDa
• 物種活性: human, monkey
• 技術: immunoprecipitation (IP): suitable; western blot: 0.5-1 μg/mL using HEK-293T total cell extract
• 同型: IgG2a
• UniProt登錄號: Q96NY9
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MUS81(80198)

描述

一般說明

Monoclonal Anti-Mus81 (mouse IgG2a isotype) is derived from the hybridoma MTA30 2G10/3 produced by the fusion of mouse myeloma cells (Sp2/0 Ag.14 cells) and splenocytes from BALB/c mice immunized with Mus81 protein. Crossover junction endonuclease Mus81 protein was identified by screening a human cerebellum cDNA library with a probe from the yeast Mus81. The human Mus81 has 551 amino acids with a molecular mass of 59 kDa. The highest sequence similarity between different species is around the VERKX3D motif. This motif is conserved in the endonuclease family (XPF) family of nucleases. The protein has helix-hairpin-helix DNA-binding domains in the N- and C-termini of the protein that are also conserved.

免疫原

Mus81 protein.

應用

Anti-Mus81 antibody, Mouse monoclonal has been used in immunofluorescence, immunoblotting and immunoprecipitation.

生化／生理作用

Crossover junction endonuclease Mus81 is elevated in cells exposed to agents that block DNA replication. Knockout mice that are homozygous Mus81-/-, or their heterozygous cohorts Mus81-/+, are susceptible to spontaneous chromosomal damage and cancer predisposition suggesting that this protein is important for genomic integrity and tumor suppression

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable