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Key Documents

L2650

Sigma-Aldrich

大豆( Glycine max )源凝集素(大豆)

peroxidase conjugate, lyophilized powder

同義詞:

SBA, 大豆凝集素

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

生物源

Glycine max (soybean)

共軛

peroxidase conjugate

化驗

85.00-100.00% (Warburg-Christian)

形狀

lyophilized powder

效力

<80 μg per mL agglutination activity

peroxidase activity

25-100 units/mg protein

成份

Protein, ~95% modified Warburg-Christian

技術

western blot: suitable

UniProt登錄號

儲存溫度

−20°C

基因資訊

soybean ... LE1(100818710)

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應用

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

生化/生理作用

SBA不是血型特异性的,但对N-乙酰-D-半乳糖胺具有亲和力。

單位定義

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

外觀

Contains sodium citrate

準備報告

由亲和纯化凝集素制备偶联物。
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

分析報告

凝集活性以 μg/ml 表示,并且在浓度为1 mg/ml、pH 6.8的磷酸缓冲盐水中进行连续稀释测定。该活性是在25℃温育1小时后,凝集2%人血型A红细胞悬浮液的最低浓度。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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存取文件庫

C Biel et al.
Archives of toxicology, 96(6), 1815-1827 (2022-04-17)
Human Precision-cut intestinal slices (hPCIS) are used to study intestinal physiology, pathophysiology, drug efficacy, toxicology, kinetics, and metabolism. However, the use of this ex vivo model is restricted to approximately a 24 h timeframe because of declining viability of the hPCIS
A simple method for the preparation of enzyme-antibody conjugates.
M J O'Sullivan et al.
FEBS letters, 95(2), 311-313 (1978-11-15)

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