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Key Documents

K3753

Sigma-Aldrich

改良 Krebs-Henseleit 缓冲液

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

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About This Item

分類程式碼代碼:
12352207

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

儲存溫度

2-8°C

一般說明

Krebs-Henseleit改良缓冲液是一种平衡盐溶液(BSS),用于细胞培养,可以为细胞提供正常细胞代谢所需的水和无机盐。此外,它提供了一个缓冲系统,以维持介质的最佳pH值,并作为灌溉、运输和稀释剂,以维持细胞内和细胞外的渗透平衡。BSS提供葡萄糖作为细胞代谢的能量来源。

應用

Krebs-Henseleit改良缓冲液已用于:

  • 采集大鼠脑组织进行离体脑切片实验
  • 测定大鼠肺动脉内皮细胞(PAEC)内钙和钠的含量
  • 采集马的子宫和前肢血管样本

數量

配制成每升培养基中含粉末 9.6 克。

重構

补充 2.1 g/L 碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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