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Merck
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Key Documents

I7526

Sigma-Aldrich

Anti-Interleukin-4 antibody produced in goat

affinity isolated antibody, lyophilized powder

同義詞:

Anti-IL-4

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About This Item

MDL號碼:
分類程式碼代碼:
51111800
NACRES:
NA.41

生物源

goat

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

lyophilized powder

物種活性

human

技術

indirect ELISA: suitable
neutralization: suitable
western blot: suitable

UniProt登錄號

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... IL4(3565)

一般說明

Interleukin-4 (IL-4) is a T cell derived chemokine that stimulates the Th2 mediated immune response and proliferation of B cells. The effects of IL-4 are mediated by two types of receptors, type I receptor consisting of IL-4Rα and common γ chain and type II receptor composed of IL-4Rα and IL-13Rα1. The signaling stimulated by IL-4 leads to activation of JAK/STAT6 and IRS-mediated PI3K/Akt pathway. Through these pathways, IL-4 is responsible for endocytic activity of macrophages, chemotaxis of leukocytes in response to inflammation, angiogenesis and regulation of nitric oxide metabolism in macrophages. Anti-tumor effects of IL-4 have been reported in cancers of breast, liver and renal cells. Anti-Interleukin-4 specifically recognizes human interleukin-4. The antibody shows no cross reactivity with rmIL-4 and rrIL-4.

特異性

The antibody will neutralize the biological activity of recombinant human IL-4, but not recombinant mouse IL-4.

免疫原

recombinant human IL-4.

應用

Anti-Interleukin-4 antibody may be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For ELISA, a working concentration of 0.5-1.0 μg/ml is recommended. The antibody may be used for immunohistochemistry of cultured cells or tissue sections at a working antibody concentration of 0.5-5 μg/ml. The antibody is suitable for neutralization assay; ND50 is 0.02-0.1 μg/ml. Anti-IL-4 antibody was used for immunohistochemistry and ELISA using cervicovaginal tissue sections and cervicovaginal extracts of Belgium white rabbits.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

外觀

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Toll-like receptors and cytokines as surrogate biomarkers for evaluating vaginal immune response following microbicide administration
Gupta SM et al
Mediators of Inflammation, doi: 10-doi: 10 (2008)
Investigational therapeutics targeting the IL-4/IL-13/STAT-6 pathway for the treatment of asthma
Oh CK et al
European Respiratory Review, 19, 11546-11554 (2010)
M Stein et al.
The Journal of experimental medicine, 176(1), 287-292 (1992-07-01)
Expression of the macrophage mannose receptor is inhibited by interferon gamma (IFN-gamma), a T helper type 1 (Th-1)-derived lymphokine. Interleukin 4 (IL-4), a Th-2 lymphocyte product, upregulates major histocompatibility class II antigen expression but inhibits inflammatory cytokine production by macrophages.
Alberto Mantovani et al.
Trends in immunology, 25(12), 677-686 (2004-11-09)
Plasticity and functional polarization are hallmarks of the mononuclear phagocyte system. Here we review emerging key properties of different forms of macrophage activation and polarization (M1, M2a, M2b, M2c), which represent extremes of a continuum. In particular, recent evidence suggests
E M Coccia et al.
International immunology, 12(7), 977-985 (2000-07-06)
NO is a labile radical involved in several immunological, antimicrobial and inflammatory processes. In macrophages, NO formation is catalyzed by the cytokine-inducible enzyme inducible NO synthase (iNOS). The importance of IFN regulatory factor (IRF)-1 and of the signal transducers and

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