H8164

Anti-dimethyl, phospho-Histone H1.4 (diMe-Lys²⁶. pSer²⁷) 兔抗

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-H1.4K26me2S27p, Anti-H1K26me2S27p
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~35 kDa
• 物種活性: human
• 濃度: ~1.5 mg/mL
• 技術: western blot: 0.5-1 μg/mL using acid-extracted fraction of HL60 cells
• UniProt登錄號: P10412
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: dimethylation (Lys26), phosphorylation (pSer27)
• 基因資訊: human ... HIST1H1E(3008)

描述

特異性

4 (diMe-Lys26, pSer27) specifically recognizes human histone H1.

免疫原

dimethylated and phosphorylated synthetic peptide corresponding to amino acids 22-33 (diMe-Lys²⁶, pSer²⁷) of human histone H1.4.

應用

Anti-dimethyl, phospho-Histone H1.4 (diMe-Lys26, pSer27) antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using acid-extracted fraction of HL60 cells.

生化／生理作用

The building block of chromatin is the nucleosome which is comprised of an octamer of core histones (H2A, H2B, H3, and H4) around which 147 bp of DNA are wrapped. In mammalian cells, four histone H1 variants (H1.2 to H1.5) are present in all somatic cells, and a fifth (H1.1) is restricted to thymus, testis, and spleen and possibly lymphocytic and neuronal cells. Histone H1.4 is di-methylated or acetylated at Lys26. Lys26 is located within the flexible N-terminal domain of H1.4, just preceding the globular domain.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)