H5041

Cystatin C human

HumanKine^®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, suitable for cell culture

• MDL號碼: MFCD00163390
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 產品名稱: Cystatin C human, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in HEK 293 cells
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized powder
• 效力: ≤5 μM IC₅₀
• 品質: endotoxin tested
• 分子量: dimer 12-13 kDa (non-glycosylated)
• 包裝: pkg of 10 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: cell culture | mammalian: suitable
• 雜質: ≤1 EU/mg
• UniProt登錄號: P01034
• 儲存溫度: −20°C
• 基因資訊: human ... CYTC(1471)

描述

一般說明

HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.

應用

Cystatin C human has been used as a standard in western blot assay. It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).

生化／生理作用

Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.

外觀

Lyophilized from a 0.2 μm filtered solution of 1×PBS

分析報告

The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC₅₀ value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.

法律資訊

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable