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重要文件

H5000

Sigma-Aldrich

己糖激酶 来源于酿酒酵母

Type III, lyophilized powder, ≥25 units/mg protein (biuret)

同義詞:

ATP:D-己糖-6-磷酸转移酶, 己糖激酶 来源于酵母

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410

種類

Type III

形狀

lyophilized powder

比活性

≥25 units/mg protein (biuret)

分子量

dimer 110 kDa

UniProt登錄號

異物活動

glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%

儲存溫度

−20°C

基因資訊

bakers yeast ... HXK1(850614) , HXK2(852639)

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生化/生理作用

以ATP作为磷酸盐来源,催化C6位的 D-己糖的磷酸化。

不同的己糖具有不同的磷酸化速率(pH 7.5,30 °C)。
D-果糖 KM:0.33 mM
D-葡萄糖 KM:0.12 mM
D-甘露糖 KM:0.05 mM

酵母己糖激酶具有两种相似的亚型,即PI和PII(A和B), 其等电点分别为5.25和4。

分子量: ~ 54 kDa (单体)
~110 kDa (二聚体)
最适pH:7.5至9.0
消光系数:在280 nm处,E1% = 8.85 (PI)和9.47 (PII)

活化剂:己糖激酶的活化需要 Mg2+ 离子(KM = 2.6 mM)。 己糖激酶可被儿茶酚胺及其相关化合物激活。

抑制剂:山梨糖-1-磷酸,多磷酸盐,6-脱氧-6-氟葡萄糖,2-C-羟基-甲基葡萄糖,木糖,来苏糖和硫醇反应性化合物(Hg2+ 和4-氯代苯甲酸酯)

單位定義

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.

外觀

Lyophilized powder containing approx. 15% sodium citrate

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Charles Betz et al.
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Markus Weiler et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(1), 409-414 (2013-12-25)
A hypoxic microenvironment induces resistance to alkylating agents by activating targets in the mammalian target of rapamycin (mTOR) pathway. The molecular mechanisms involved in this mTOR-mediated hypoxia-induced chemoresistance, however, are unclear. Here we identify the mTOR target N-myc downstream regulated
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Daniel Romaker et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6335-6340 (2014-04-16)
MicroRNAs (miRNAs) are major posttranscriptional regulators of a wide variety of biological processes. However, redundancy among most miRNAs has made it difficult to identify their in vivo functions. We previously demonstrated that global inhibition of miRNA biogenesis in Xenopus resulted
Seok-Hyung Kim et al.
PLoS genetics, 9(6), e1003563-e1003563 (2013-06-21)
Multiple Acyl-CoA Dehydrogenase Deficiency (MADD) is a severe mitochondrial disorder featuring multi-organ dysfunction. Mutations in either the ETFA, ETFB, and ETFDH genes can cause MADD but very little is known about disease specific mechanisms due to a paucity of animal

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