H0273

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse

clone IAP-28, ascites fluid

• MDL號碼: MFCD02189139
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: IAP-28, monoclonal
• 分子量: antigen 25 kDa
• 包含: 15 mM sodium azide
• 物種活性: chicken, bovine, frog, rabbit, guinea pig, turkey, Drosophila, mouse, rat, human
• 技術: immunohistochemistry: suitable using frozen sections of chicken gizzard smooth muscle; indirect ELISA: suitable; indirect immunofluorescence: suitable using formalin-fixed, heat-shocked cultured chick embryo fibroblasts; radioimmunoassay: suitable; western blot: 1:500 using turkey gizzard extract
• 同型: IgG1
• UniProt登錄號: P04792
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HSPB1(3315) ; mouse ... Hspb1(15507) ; rat ... Hspb1(24471)

描述

一般說明

Monoclonal anti-Heat Shock Protein 25 (mouse IgG1) isotype) is derived from the IAP-28 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle. Heat shock (stress) proteins (HSPs) are a group of polypeptides synthesized in response to a wide variety of environmental factors, including biotic and abiotic stressors. These proteins are produced by prokaryotic and eukaryotic cells, and HSPs are among the most conserved molecules in phylogeny. Heat shock protein 25 is encoded by the gene mapped to human chromosome 7q11.23. The encoded protein belongs to the class of small HSPs. Small HSPs aggregate to form characteristic ring-shaped structures called heat shock granules. Small HSPs contains a-crystallin domain” (ACD) C-terminal domain, N-terminal domain and C-terminal extension (CTE).

特異性

The antibody also reacts with a dimer of the HSP 25 (approx. 58 kDa) appearing in preparations stored for prolonged periods prior to electrophoretic analysis. A major proteolytic fragment of 22 kDa may also be detected. The antibody suppresses the inhibition of actin elongation induced by the 25 kDa IAP. The antibody displays a punctate or fine fibrillary pattern of labeling throughout the cytoplasm in heat-shocked cells and co-localization with mitochondria. Reactivity has been observed with HSP 25 of various organs.

免疫原

partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.

應用

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• radioimmunoassay (RIA)
• immunocytochemistry
• immunohistofluorescence assay
• immunopurification

生化／生理作用

Small heat shock proteins (HSPs) play a vital role in stress tolerance. They act as molecular chaperones in vitro. Murine Hsp25 averts aggregation of citrate synthase and α-glucosidase. It also inhibits actin polymerization and the amyloid fibril formation of Aβ1-42. Mutations in human sHsps result in myopathies, neuropathies and cataract. The small HSPs have also been implicated in phenomena of acquired thermotolerance and neoplasia.

外觀

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

儲存和穩定性

For continuous use, store at 2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable