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H0262

Sigma-Aldrich

Hybri-Max™ HAT培养基补充物 (50×)

lyophilized powder, suitable for hybridoma, BioXtra

同義詞:

HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

Hybri-Max™ HAT培养基补充物 (50×), γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

無菌

γ-irradiated

產品線

BioXtra

形狀

lyophilized powder

技術

cell culture | hybridoma: suitable

雜質

endotoxin, tested

運輸包裝

dry ice

儲存溫度

−20°C

應用

HAT培养基添加剂(50×) Hybri-Max 已用于:
  • 次黄嘌呤-鸟嘌呤磷酸核糖基转移酶 (HPRT) 缺陷型克隆体的选择和验证
  • mAbs(单克隆抗体)制备
  • 基于荧光素酶的生物测定 (LUC)

重構

用 10 ml 无菌细胞培养基重悬小瓶的内容物。 储备溶液足以制备 500 ml 培养基。 最终工作浓度:100 μM 氨基喋呤, 0.4 μM 氨基喋呤,16 μM 胸苷

法律資訊

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 4 Oral - Repr. 1B

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges


分析證明 (COA)

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Geoffrey A Mueller et al.
Journal of immunology (Baltimore, Md. : 1950), 205(8), 1999-2007 (2020-09-11)
IgE Abs drive the symptoms of allergic disease upon cross-linking allergens on mast cells or basophils. If the IgE binding sites on the allergens could be identified, it may be useful for creating new forms of immunotherapy. However, direct knowledge
The expression and function of the NKRP1 receptor family in C57BL/6 mice
Aust J G, et al.
Journal of immunology (Baltimore, Md. : 1950), jimmunol-j0804281 (2009)
The expression and function of the NKRP1 receptor family in C57BL/6 mice
Aust J G, et al.
Journal of Immunology, jimmunol-j0804281 (2009)
Youngkyun Kim et al.
Clinical & translational immunology, 4(11), e51-e51 (2015-12-19)
This study was undertaken to develop a novel anti-citrullinated peptide antibody (ACPA) and to investigate its arthritogenicity in a collagen-induced arthritis (CIA) model. The novel ACPA, 12G1, was developed by injecting cyclic citrullinated antigen in mice and subsequently hybridizing the
Development and validation of cell-based luciferase reporter gene assays for measuring neutralizing anti-drug antibodies against interferon beta
Hermanrud C, et al.
Journal of Immunological Methods, 1-9 (2016)

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