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GLN1

Sigma-Aldrich

谷氨酰胺和谷氨酸检测试剂盒

同義詞:

L-glutamine enzymatic quantification kit

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

用途

 kit sufficient for 50 tests

品質等級

200

包裝

pkg of 1 kit

儲存條件

dry at room temperature

技術

UV/Vis spectroscopy: suitable

λmax

340 nm

應用

cell analysis
detection

儲存溫度

2-8°C

相關類別

應用

该试剂盒通过将NAD还原为NADH，在339 nm波长处测定分光光度，用酶法测定L-谷氨酰胺和L-谷氨酸，
检测范围为：0.056-0.56 mM L-谷氨酸；0.14-1.4 mM L-谷氨酰胺。
谷氨酰胺和谷氨酸测定试剂盒用于确定以下物质中L-谷氨酰胺的浓度：
  • 最低必需培养基（MEM）培养基
  • 上清液
  • 血浆

生化／生理作用

谷氨酰胺/谷氨酸测定试剂盒设计通过L-谷氨酰胺酶促脱氨、L-谷氨酸脱氢以及NAD+ 转为NADH后，用分光光度法测量L-谷氨酰胺和/或L-谷氨酸。该试剂盒可用于分光光度法测定液体制剂中的L-谷氨酰胺和/或L-谷氨酸。

訊號詞

Danger

危險分類

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Tianyu Han et al.
Cell research, 28(6), 655-669 (2018-03-09)
Glutamine metabolism plays an important role in cancer development and progression. Glutaminase C (GAC), the first enzyme in glutaminolysis, has emerged as an important target for cancer therapy and many studies have focused on the mechanism of enhanced GAC expression
Yingchao Lin et al.
BMC plant biology, 17(1), 247-247 (2017-12-21)
The trehalose (Tre) pathway has strong effects on growth and development in plants through regulation of carbon metabolism. Altering either Tre or trehalose 6-phosphate (T6P) can improve growth and productivity of plants as observed under different water availability. As yet
Expansion of human mesenchymal stromal cells on microcarriers: growth and metabolism
Schop D, et al.
Journal of Tissue Engineering and Regenerative Medicine, 4(2), 131-140 (2010)
Maryvonne Ardourel et al.
Nutrients, 13(9) (2021-09-29)
To prevent ocular pathologies, new generation of dietary supplements have been commercially available. They consist of nutritional supplement mixing components known to provide antioxidative properties, such as unsaturated fatty acid, resveratrol or flavonoids. However, to date, only one preclinical study
I Made Adi Parmana et al.
Vascular health and risk management, 18, 219-231 (2022-04-13)
Myocardial injury due to on-pump coronary artery bypass grafting (CABG) in patients with low ejection fraction (EF) is associated with poor outcomes. This study determines whether intravenous glutamine could protect the myocardium during on-pump CABG in patients with low EF.
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