推薦產品
包裝
pkg of 1 kit
製造商/商標名
Cytiva RPN5785
顏色
ECF substrate: Off-white/white to yellowish ECF substrate dilution buffer: Colorless
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
Alkaline phosphatase dephosphorylates ECF™ Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.
The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF™ Substrate, 60 mL, and dilution buffer.
The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF™ Substrate, 60 mL, and dilution buffer.
應用
Vistra ECF™ substrate has been used in Western blotting.
特點和優勢
- A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm)
- For use in western blotting, dot, and slot blotting applications
- Sensitivity comparable to chemiluminescence
- The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
- Eliminates need for darkroom and film
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
ECF is a trademark of Cytiva
訊號詞
Danger
危險聲明
儲存類別代碼
12 - Non Combustible Liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
Anti-atherosclerotic effects of small molecular weight compounds enhancing eNOS expression and preventing eNOS uncoupling.
Journal of Pharmacology and Experimental Therapeutics (2007)
PloS one, 9(2), e87367-e87367 (2014-03-04)
Poly(ADP-ribose)polymerase and sirtuin 1 are both NAD(+)-dependent enzymes. In vitro oxidative stress activates poly(ADP-ribose)polymerase, decreases NAD(+) level, sirtuin 1 activity and finally leads to cell death. Poly(ADP-ribose)polymerase hyperactivation contributes to cell death. In addition, poly(ADP-ribose)polymerase inhibition restores NAD(+) level and
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