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Key Documents

GE28-9505-11

HiScreen™ Q High Performance

Cytiva 28-9505-11

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About This Item

分類程式碼代碼:
47101511
NACRES:
NA.56

ligand

quaternary amine

描述

Ion Exchanger Type (value)

包裝

pkg of 1 ea

製造商／商標名

Cytiva 28-9505-11

參數

44 psi

床尺寸

7.7 mm × 100 mm

床體積

4.7 mL

柱內徑

7.7 mm

基質

6% cross-linked agarose

粒徑

24-44 μm

樣品平均尺寸

34 μm

cleaning

1-14

工作範圍

2-12

適合性

suitable for bioprocess medium

一般說明

HiScreen™ Q HP columns are prepacked with Q Sepharose™ High Performance ion exchange chromatography media and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

應用

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ IEX HP columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

特點和優勢

  • Q Sepharose™ High Performance is a strong anion exchange BioProcess medium designed for intermediate and polishing steps in downstream processing
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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文章

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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條款

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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