GE28-4009-89

His MultiTrap^™ High Performance

Cytiva 28-4009-89, pack of 4 plates

• 同義詞: High Performance His Trap, His MultiTrap, MultiTrap for His
• 分類程式碼代碼: 12161501
• NACRES: NA.32

屬性

• 材料: polypropylene and polyethylene
• 包裝: pack of 4 plates
• 製造商／商標名: Cytiva 28-4009-89
• 尺寸: 127.8 mm × 85.5 mm × 30.6 mm
• 基質: highly cross-linked 6% agarose
• 樣品平均尺寸: 34 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• 工作範圍: 3-12(Ni²⁺-stripped medium.)
• 容量: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

描述

一般說明

His MultiTrap^™ FF and His MultiTrap^™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).

應用

His MultiTrap^™ FF and His MultiTrap^™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose^™ 6 Fast FLow (His MultiTrap^™ FF) or Ni Sepharose^™ High Performance (His MultiTrap^™ HP). A protocol for His MultiTrap^™ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.

Loading unclarified samples directly to His MultiTrap^™ FF and His MultiTrap^™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap^™ FF and His MultiTrap^™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.

特點和優勢

• Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
• Increased convenience and consistency with prepacked 96-well plates.
• No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
• Use His MultiTrap^™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
• Easy scale-up to HisTrap^™ FF, HisPrep^™ FF 16/10 or HisTrap^™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HisPrep is a trademark of Cytiva

HisTrap is a trademark of Cytiva

MultiTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS09
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids