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G7402

Sigma-Aldrich

抗兔 IgG(全分子)-金 山羊抗

affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

品質等級

共軛

gold conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

aqueous glycerol suspension

存貨情形

not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)

技術

dot blot: suitable
western blot: suitable

粒徑

10 nm (colloidal gold)

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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相關類別

一般說明

IgG 抗体在补体激活、吞噬作用、胎盘转运和细胞表面受体结合等体液免疫应答中起关键作用。抗兔IgG(全分子)-金抗体可用作二抗(在含有1% BSA和5% NGS的0.01 MPBS中以1:50稀释),通过包埋后实验对GABA和谷氨酸进行EM免疫标记。它也可用于改进的点印迹试验,以确定结合活性。山羊抗兔IgG(全分子)-金抗体与所有山羊免疫球蛋白发生特异性反应。
IgG 是糖蛋白抗体,可调节几种免疫反应。针对靶蛋白的兔 IgG 常被用作各种研究应用的一抗。因此,二级抗兔IgG可作为分析靶蛋白的有用工具。
抗兔IgG(全分子)-金抗体是兔IgG的相应抗体。

免疫原

从正常兔血清中纯化的兔 IgG。

應用

成功使用该抗体的应用以及相关同行评审论文如下所示。
免疫电镜
免疫组化(1篇)
抗兔IgG(全分子)-金抗体(1:15稀释)可用于免疫电子显微镜。它还可用于蛋白印迹分析。

外觀

溶于含 30% 甘油 (v/v)、1% 牛血清白蛋白 (w/v) 和 15 mM 叠氮化钠的 Tris 缓冲盐水 (pH 8.2) 胶体混悬液。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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存取文件庫

Ignaz Wessler et al.
PloS one, 11(6), e0156886-e0156886 (2016-06-11)
The worldwide use of neonicotinoid pesticides has caused concern on account of their involvement in the decline of bee populations, which are key pollinators in most ecosystems. Here we describe a role of non-neuronal acetylcholine (ACh) for breeding of Apis
S Sinnarajah et al.
FEBS letters, 426(3), 377-380 (1998-05-26)
The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence
Andrea González et al.
The American journal of tropical medicine and hygiene, 92(5), 887-897 (2015-03-12)
Trypanosoma cruzi calreticulin (TcCRT), a 47-kDa chaperone, translocates from the endoplasmic reticulum to the area of flagellum emergence. There, it binds to complement components C1 and mannan-binding lectin (MBL), thus acting as a main virulence factor, and inhibits the classical
Jiongjiong Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(32), 11436-11441 (2008-08-06)
Hybrid sterility is a major form of postzygotic reproductive isolation. Although reproductive isolation has been a key issue in evolutionary biology for many decades in a wide range of organisms, only very recently a few genes for reproductive isolation were
Vladimir Varga et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(32), E6546-E6555 (2017-07-21)
The distal end of the eukaryotic flagellum/cilium is important for axonemal growth and signaling and has distinct biomechanical properties. Specific flagellum tip structures exist, yet their composition, dynamics, and functions are largely unknown. We used biochemical approaches to identify seven

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